C-C motif chemokine 2 (Protein name
), CCL2_HUMAN from NCBI database.
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General Annotation
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Antigen Annotation
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3D
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Predicted Eptitope
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Vaild Sequence
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Related Databases
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Gene name:
CCL2(MCP1;SCYA2);
Protein name:
C-C motif chemokine 2;
Alternative:
Monocyte chemoattractant protein 1;HC11;Monocyte chemotactic protein 1(MCP-1);Monocyte chemotactic and activating factor(MCAF);Small-inducible cytokine A2;Monocyte secretory protein JE;
Organism:
Human (Homo sapiens).
General Annotation
Sub Unit:
Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans.
Function:
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.3%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.5%
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Recovery
Recovery was determined by spiking various levels of CCL2 into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
97
91-99
Plasma
96
94-103
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CCL2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
92-98%
96-103%
97-105%
102-108%
EDTA plasma(n=5)
96-101%
98-105%
99-106%
91-96%
heparin plasma(n=5)
90-103%
91-104%
94-105%
95-102%
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: PROTEIN SEQUENCE OF 24-99;PYROGLUTAMATE FORMATION AT GLN-24
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Cited for: GAG BINDING SITES LYS-81 AND HIS-89;MUTAGENESIS OF LYS-81; HIS-89 AND 95-GLN--THR-99
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: HOMODIMERIZATION;MUTAGENESIS OF PRO-31; VAL-32; THR-33 AND TYR-36
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: MUTAGENESIS OF 25-PRO--PRO-31; ASP-26; ILE-28; ASN-29; PRO-31; VAL-32 AND THR-33
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: IMPORTANCE OF TYR-36; ARG-47; LYS-58; LYS-61 AND LYS-72 FOR RECEPTOR BINDING;MUTAGENESIS
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: GAG BINDING SITES ARG-41; LYS-42 AND ARG-47;MUTAGENESIS
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Cited for: INVOLVEMENT IN MYCOBACTERIUM TUBERCULOSIS SUSCEPTIBILITY
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